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Choose Expedited for fastest shipping! However, the author and publisher cannot be. It is assumed that before any. Front cover: The molecular structure is that of the hexameric form of the human hormone insulin, grouped around two zinc. The protein hexamer is thought to be the form in which insulin is stored in the beta cells of the pancreas and secreted. Rodney Boyer. Biochemistry lab courses provide students the knowledge and skills needed for future research participation at the undergraduate and graduate level, and for jobs in the biotechnological and pharmaceutical industry.

The purpose of this book is to serve as a resource to enhance student learning of theories, techniques, and methodologies practiced in the biochemistry teaching and research lab.

Biochemistry Laboratory: Modern Theory and Techniques: International Edition

The extensive availability of laboratory experiments published in journals and the desire of instructors to design their own projects and teaching styles have lessened the need for laboratory manuals. However, because published experiments and homemade lab manuals usually contain only procedures, there is an increased need for a companion text like this one to explain the theories and principles that underpin laboratory activities.

The historical development of general techniques is explored and followed by discussion of current applications Chapters 1—3. Chapters 4—11 provide an introduction to the core techniques and instrumentation that may be applied to the study of all biomolecules and biological processes: centrifugation, chromatography, electrophoresis, spectroscopy, ligand- protein binding, methods in molecular biology, protein purification, and Internet databases.

An important premise in this section is that the expansion of our knowledge in biochemistry and related molecular life sciences is dependent upon the continued development of powerful analytical techniques, especially instrumentation and computers.

The book has a Companion Website that is composed of three appendices. The first appendix is an introduction to teaching the biochemistry lab. Topics covered include a discussion of different teaching methods and the concepts and skills that should be part of a biochemistry laboratory course. The second appendix is a listing of proven experiments and projects that have been published in biochemistry education journals.

The list gives instructors the opportunity to select laboratory exercises that are compatible with their backgrounds and expertise and with available instrumentation and facilities. Special efforts were made to include projects integrating traditional topics in biochemistry with the modern topics of genomics and proteomics. The current list has approximately experimental projects and will be updated on a periodic basis.

The third appendix is a listing of useful Web sites for each chapter, which will be updated as necessary.